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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Agroenergia. |
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Data corrente: |
12/09/2017 |
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Data da última atualização: |
15/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BASSO, M. F.; DIAS, B. B. A.; RIBEIRO, A. P.; MARTINS, P. K.; SOUZA, W. R.; OLIVEIRA, N. G. DE.; NAKAYAMA, T. J.; CASARI, R. A das C. N.; SANTIAGO, T. R.; VINECKY, F.; JUNGMANN, L.; SOUSA, C. A. F. de; OLIVEIRA, P. A. de; SOUZA, S. A. C. D. de; CANCADO, G. M. de A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. |
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Afiliação: |
MARCOS FERNANDO BASSO; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ANA PAULA RIBEIRO; POLYANA KELLY MARTINS; WAGNER RODRIGO DE SOUZA; NELSON GERALDO DE OLIVEIRA; THIAGO JONAS NAKAYAMA; RAPHAEL AUGUSTO DAS CHAGAS NOQUELI CASARI; THAIS RIBEIRO SANTIAGO; FELIPE VINECKY; LETICIA JUNGMANN CANCADO, CNPAE; CARLOS ANTONIO FERREIRA DE SOUSA, CNPAE; PATRICIA ABRAO DE OLIVEIRA, CNPAE; SILVANA APARECIDA CRESTE DIAS DE SOUZA; GERALDO MAGELA DE ALMEIDA CANCADO, CNPTIA; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE. |
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Título: |
Improved genetic transformation of sugarcane (Saccharum spp.) embryogenic callus mediated by Agrobacterium tumefaciens. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Current Protocols in Plant Biology, v. 2, n. 3, p. 221-239, Sept. 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Sugarcane (Saccharum spp.) is a monocotyledonous semi-perennial C4 grass of the Poaceae family. Its capacity to accumulate high content of sucrose and biomass makes it one of the most important crops for sugar and biofuel production. Conventional methods of sugarcane breeding have shown several limitations due to its complex polyploid and aneuploid genome. However, improvement by biotechnological engineering is currently the most promising alternative to introduce economically important traits. In this work, we present an improved protocol for Agrobacterium tumefaciens-mediated transformation of commercial sugarcane hybrids using immature top stalk-derived embryogenic callus cultures. The callus cultures are transformed with preconditioned A. tumefaciens carrying a binary vector that encodes expression cassettes for
a gene of interest and the bialaphos resistance gene (bar confers resistance to glufosinate-ammonium herbicide). This protocol has been used to successfully transform a commercial sugarcane cultivar, SP80-3280, highlighting: (i) reduced recalcitrance and oxidation; (ii) high yield of embryogenic callus; (iii) improved selection; and (iv) shoot regeneration and rooting of the transformed plants. Altogether, these improvements generated a transformation efficiency of 2.2%. This protocol provides a reliable tool for a routine procedure for sugarcane improvement by genetic engineering. |
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Palavras-Chave: |
Agrotransformation; Cultivar SP80-3280; Immature top stalks; Transgenic bioenergy crops. |
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Thesagro: |
Agrobacterium Tumefaciens; Biomassa; Cana de açúcar; Engenharia genética. |
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Thesaurus Nal: |
Agrobacterium radiobacter; Genetic engineering; Saccharum; Sugarcane. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02799naa a2200457 a 4500
001 2075481
005 2018-01-15
008 2017 bl uuuu u00u1 u #d
100 1 $aBASSO, M. F.
245 $aImproved genetic transformation of sugarcane (Saccharum spp.) embryogenic callus mediated by Agrobacterium tumefaciens.$h[electronic resource]
260 $c2017
520 $aSugarcane (Saccharum spp.) is a monocotyledonous semi-perennial C4 grass of the Poaceae family. Its capacity to accumulate high content of sucrose and biomass makes it one of the most important crops for sugar and biofuel production. Conventional methods of sugarcane breeding have shown several limitations due to its complex polyploid and aneuploid genome. However, improvement by biotechnological engineering is currently the most promising alternative to introduce economically important traits. In this work, we present an improved protocol for Agrobacterium tumefaciens-mediated transformation of commercial sugarcane hybrids using immature top stalk-derived embryogenic callus cultures. The callus cultures are transformed with preconditioned A. tumefaciens carrying a binary vector that encodes expression cassettes for a gene of interest and the bialaphos resistance gene (bar confers resistance to glufosinate-ammonium herbicide). This protocol has been used to successfully transform a commercial sugarcane cultivar, SP80-3280, highlighting: (i) reduced recalcitrance and oxidation; (ii) high yield of embryogenic callus; (iii) improved selection; and (iv) shoot regeneration and rooting of the transformed plants. Altogether, these improvements generated a transformation efficiency of 2.2%. This protocol provides a reliable tool for a routine procedure for sugarcane improvement by genetic engineering.
650 $aAgrobacterium radiobacter
650 $aGenetic engineering
650 $aSaccharum
650 $aSugarcane
650 $aAgrobacterium Tumefaciens
650 $aBiomassa
650 $aCana de açúcar
650 $aEngenharia genética
653 $aAgrotransformation
653 $aCultivar SP80-3280
653 $aImmature top stalks
653 $aTransgenic bioenergy crops
700 1 $aDIAS, B. B. A.
700 1 $aRIBEIRO, A. P.
700 1 $aMARTINS, P. K.
700 1 $aSOUZA, W. R.
700 1 $aOLIVEIRA, N. G. DE.
700 1 $aNAKAYAMA, T. J.
700 1 $aCASARI, R. A das C. N.
700 1 $aSANTIAGO, T. R.
700 1 $aVINECKY, F.
700 1 $aJUNGMANN, L.
700 1 $aSOUSA, C. A. F. de
700 1 $aOLIVEIRA, P. A. de
700 1 $aSOUZA, S. A. C. D. de
700 1 $aCANCADO, G. M. de A.
700 1 $aKOBAYASHI, A. K.
700 1 $aMOLINARI, H. B. C.
773 $tCurrent Protocols in Plant Biology$gv. 2, n. 3, p. 221-239, Sept. 2017.
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